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KMID : 0603820100160020091
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Journal of Experimental & Biomedical Science
2010 Volume.16 No. 2 p.91 ~ p.95
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Anti-inflammatory Properties of Meso-dihydroguaiaretic Acid in Lipopolysaccharide-induced Macrophage
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Kim Yong-Jae
Kang Yeo-Jin
Kim Tack-Joong
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Abstract
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Meso-dihydroguaiaretic acid (MDGA) is a medicinal herbal product isolated from the bark of Machilus thunbergii Sieb. et Zucc. (Lauraceae). It exhibits a neuroprotective effect and also exerts cytotoxicity to certain cancer cells. In the present study, we investigated whether or not MDGA inhibits inflammatory reaction through the inhibition of nitric oxide (NO) generation. The results showed that MDGA (5~25 μM) inhibited 100 ng/㎖ lipopolysaccharide (LPS)-induced NO generation in macrophage Raw 264.7 cells in a concentration-dependent manner. We also measured the cytotoxic effects of MDGA on Raw 264.7 cells and found no evidence of cytotoxicity. The inhibition of NO generation by MDGA was consistent with the inhibitory effect on the expression of inducible nitric oxide synthase (iNOS). In addition, MDGA inhibited the LPS-induced gene expression of interleukin-1β (IL-1β) as well as tumor necrosis factor-α (TNF-α). The present results may provide that MDGA has anti-inflammatory properties through inhibition of the toll-like receptors (TLRs) pathway, and suggest that MDGA can be used as an anti-inflammatory agent.
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KEYWORD
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Meso-dihydroguaiaretic acid, Toll-like receptor, Nitric oxide
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